David Rozema TIDES Conference Workshop 3

[partimefriend]

May 9-12 ,  2016

Protease-sensitive Endosomal Escape for Delivery of siRNA in vivo

We have developed a mechanism for control of cytoplasmic release which is based on reversible inhibition of membrane interactive polymers with protease-sensitive substrates that inhibit non-specific membrane interactions. Upon hydrolysis of these substrates by intracellular proteases, membrane activity of the polymers is restored, thereby allowing cytoplasmic access of siRNA.
David Rozema, Ph.D., Vice President, Arrowhead Research Corporation

If you go to the web site in workshop #3  to the right they have NEW DATA Highlighted in blue. 


www.ibclifesciences.com/TIDES/workshops.xml#w3

www.sciencedirect.com/science/article/pii/S0168365915002308?via%3Dihub

[qbeing1010]

Sounds great. Can someone explain this in layman's terms?

[partimefriend]

Also found this at workshop 4 

Workshop #4:
Bioanalysis and DMPK of Oligonucleotides and Peptides/Proteins

Sensitive and Specific Quantification of Oligonucleotides in Biological Matrices using Hybridization Based LC-fluorescence Assays
Qingguo Tian, Ph.D., Bioanalytical Project Manager, Arrowhead Research

[holdencf]

Wayne
How does the siRNA get into the nucleus once in the cytoplasm.  Does it enter via the Nuclear Pore Complex which would be the same way a virus (HBV) gets into the nucleus?  As I understand it. of the 4-5 ways for a molecule to enter the nucleus the only way for it to enter without damaging the nucleus is via the NPC.  

[end2war]

It appears that this will be a discussion of progress being made on SQ administration of RNAi. Very important!!